DNA Vectors
System Biosciences LLC Cre Recombinase Expression Plasmid (pCMV-CRE) | 10ug
With the Cre-LoxP site-specific recombination system used in most of our HR Targeting Vectors, you can get rid of extra sequences, such as selection cassettes, by transiently expressing the Cre recombinase from the pCMV-CRE Cre Recombinase Expression Plasmid.
Vector Biolabs Enhancer of zeste homolog 2 Adenovirus (Ad-EZH2)
This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. This protein may play a role in the hematopoietic and central nervous systems. Two transcript variants encoding distinct isoforms have been identified for this gene.
Vector Biolabs p21/CDKN1A Adenovirus (Ad-CMV-p21)
p21/CDKN1A is a cyclin-dependent kinase inhibitor. It is a key effector of cellular senescence downstream of the tumor suppressor p53. p21 binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of p21/CDKN1A is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. P21 can interact with proliferating cell nuclear antigen (PCNA), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation.
Vector Biolabs Rabaptin, RAB GTPase binding effector protein 2 Adenovirus (Ad-SERCA1)
Rabaptin, RAB GTPase binding effector protein 2 Adenovirus (Ad-SERCA1)
Vector Biolabs FOXO3A (DN) Adenovirus (Ad-CMV-FOXO3A (DN))
The dominant-negative form (dn-FOXO3a) was constructed by deletion of the transactivation domain from the C terminus.
Vector Biolabs Interleukin 1, alpha Adenovirus (Ad-IL1(alpha))
The interleukins are a broad family of well characterized cytokines, primarily of hematopoietic cell origin. As new cytokines are molecularly characterized, they are assigned an IL number to maintain a standard nomenclature. The interleukins are secreted by immune cells (mainly macrophages, B-cells or T-cells) that regulate a wide range of immune system functions. The functions of different interleukins vary from regulation inflammatory and immune responses, functioning as autocrine factor and regulation and/or inhibition of other interleukins.
Vector Biolabs Suppressor of cytokine signaling 3 Adenovirus (Ad-SOCS3)
This gene encodes a member of the STAT-induced STAT inhibitor (SSI), also known as suppressor of cytokine signaling (SOCS), family. SSI family members are cytokine-inducible negative regulators of cytokine signaling. The expression of this gene is induced by various cytokines, including IL6, IL10, and interferon (IFN)-gamma. The protein encoded by this gene can bind to JAK2 kinase, and inhibit the activity of JAK2 kinase. Studies of the mouse counterpart of this gene suggested the roles of this gene in the negative regulation of fetal liver hematopoiesis, and placental development.
Vector Biolabs FOXO3A (AAA) Adenovirus (Ad-CMV-FKHRL1 (AAA))
The FKHRL1-AAA triple mutant is not phosphorylatable because three phosphorylation sites, Thr32, Ser253, and Ser315, were replaced by alanine residues.
Vector Biolabs human SIRT1 shRNA Adenovirus (Ad-h-SIRT1-shRNA)
This is an pre-made gene silencing adenovirus that expresses a shRNA to knockdown human SIRT 1 gene. The shRNA expression is driven by an U6 promoter.
The knockdown of this human gene was validated by qPCR in A549 cells.
Vector Biolabs vascular endothelial growth factor D Adenovirus (Ad-VEGF-D)
The onset of angiogenesis is believed to be an early event in tumorigenesis and may facilitate tumor progression and metastasis. Several growth factors with angiogenic activity have been described. These include FGF, PDGF, vascular endothelial growth factor (VEGF), VEGF-B, VEGF-C, VEGF-D (or FIGF), EG-VEGF and placenta growth factor (PlGF). VEGF-D undergoes a complex proteolytic maturation, generating multiple processed forms which bind and activate VEGFR-2 and VEGFR-3 receptors. This protein is structurally and functionally similar to vascular endothelial growth factor C.
Vector Biolabs AAV-RFP-iCre (AAV1) AAV (AAV1-RFP-iCre)
Cre recombinase is used as a tool to genetically modify genes, such as to delete a segment of DNA flanked by LoxP sites in cells or experimental animals.By applying the mammalian codon usage to Cre recombinase, expression of Cre is improved in the mammalian cells. This improved Cre (iCre) gene also reduce the high CpG content of the prokaryotic coding sequence, thereby reducing the chances of epigenetic silencing in mammals.This AAV1 stock expresses an improved Cre (iCre) along with a RFP under a control of the same CMV promoter. The Cre and RFP was separated by a 2A peptides.
Vector Biolabs FLAG tagged CRISPR/Cas9 nuclease RGD-fiber modified Adenovirus (Ad(RGD)-mCherry-FLAG-hCas9)
The Type II prokaryotic CRISPR/Cas system is the new class of tools for targeted genome engineering. The cas9 nucleases derived from clustered regularly interspaced short palindromic repeats (CRISPR)-cas systems uses small RNAs as guides (gRNA) to cleave DNA in a sequence-specific manner. With its ease in designing guide sequences to target specific genomic loci, the CRISPR/Cas system is a much simpler, faster, and robust alternative to TALEN and Zinc finger nuclease platforms. For site-specific genome editing, the CRISPR/Cas9 system requires the Cas 9 nuclease and the gRNA. This RGD-fiber modified adenovirus expresses a codon-optimized Cas9 nuclease with N-terminal FLAG tag and a mCherry reporter. It can be used along with guided RNA for genome-editing in cells that regular Ad5 doesn't infect well, including human or mouse macrophages, ES cells, T cells, synoviocytes, certain fibroblast and islet grafts etc.
Vector Biolabs caveolin 1, caveolae protein, 22kDa Adenovirus (Ad-Caveolin-1)
The scaffolding protein encoded by this gene is the main component of the caveolae plasma membranes found in most cell types. The protein links integrin subunits to the tyrosine kinase FYN, an initiating step in coupling integrins to the Ras-ERK pathway and promoting cell cycle progression. The gene is a tumor suppressor gene candidate and a negative regulator of the Ras-p42/44 MAP kinase cascade. CAV1 and CAV2 are located next to each other on chromosome 7 and express colocalizing proteins that form a stable hetero-oligomeric complex. By using alternative initiation codons in the same reading frame, two isoforms (alpha and beta) are encoded by a single transcript from this gene.
Vector Biolabs Calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha Adenovirus (Ad-CaMKII(alpha))
Ca2+/calmodulin-dependent protein kinases (CaM kinases) compose a structurally related subfamily of serine/threonine kinases. Members of this family include phosphorylase kinase, myosin light chain kinases (MLCKs) and CaM kinases (CaMKs) I, II, III and IV. Of these, CaMKII comprises of four distinct subunits, alpha, beta, gamma and delta. Upstream regulators of CaMKI and CaMKIV, designated CaMKK and CaMKK(beta), function to activate CaMKI by specific phosphorylation at threonine 177. MLCK isoforms include non-muscle MLCK, smooth muscle MLCK and skeletal muscle MLCK (MLCKSK).